Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2018 , Vol 24 , Issue 5
Development and Evaluation of an ELISA System for Detection of PEDV-Specific IgA Antibodies in Colostrum
Ren-Feng LI1, Xiang-Qin TIAN2, Yan YU1, Jin-Qing JIANG1, San-Hu WANG1, Zi-Liang WANG1, Jin-You MA1, Li-Rong WANG1, Shou-Ping ZHANG1, Yan-Yan YANG3
1College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, CHINA
2Medical Research Center, Xinxiang Medical College, Xinxiang 453003, CHINA
3Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, CHINA
DOI : 10.9775/kvfd.2018.19776 Porcine epidemic diarrhea virus (PEDV) is a devastating swine infectious disease. Development of high-performance methods to diagnose and evaluate viral immune status remains very important to control PEDV. Here, a recombinant S1 protein-based indirect enzyme-linked immunosorbent assay (rpS1-iELISA) was developed to monitor IgA antibody in the colostrum. Optimized cut-off value of the rpS1-iELISA was determined as 0.448. Results yielded a sensitivity of 96.87% and a specificity of 100.0%. Repeatability tests indicated that the coefficients of variation of the colostrum samples within and between runs were both less than 8%. Test results of 523 field colostrum samples showed that the rpS1-iELISA had excellent agreement with immunofluorescence assay (kappa = 0.958) and better test performance than a commercial ELISA kit. This test will aid in future diagnostics and assessment of the protective levels of mucosal immune response against PEDV by measuring IgA levels in the colostrum. Keywords : ELISA, IgA antibody, Colostrum, Porcine epidemic diarrhea virus, Spike protein