¹College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, CHINA DOI : 10.9775/kvfd.2018.21007 The purpose of this study was to establish the model of canine deep layer corneal ulcer by means of alkali burn combined with mechanical injury and evaluate the efficacy of conjunctival covering combined with amnion liquid supernatant deep layer corneal ulcer in beagles. By using alkali burnand mechanical injury establishd the beagle deep layer corneal ulcer model (n=15) , randomly divided into 3 groups: deep layer corneal ulcer (C group), conjunctival covering group (T1 group), conjunctival covering combined amnion liquid supernatant eyedrop group (T2 group). On -3, 1, 7, 14, 21, 45 days, examine the levels of Interleukin -1 (IL-1), Interleukin-8 (IL-8), Vascular Endothelial Growth Factor (VEGF), Matrix metalloproteinase-1 (MMP-1), Matrix metalloproteinase-2 (MMP-2) and Matrix metalloproteinase-9 (MMP-9) in canine aqueous humor; On the 45th day, two experimental canines were randomly selected from each group to collect corneal tissue for histopathological observation. C group compared to groups T1 group and T2 group , the levels of IL-1, IL-8, VEGF, MMP-1, MMP-2, MMP-9 in canine aqueous humor significantly decreased (P<0.05). Histopathology revealed that in the T2 group corneal epithelial blood vessels were less, corneal thickness was moderate, the cornea was more complete, the cornea had fewer blood vessels, and the inflammatory cell infiltration was lower. Conjunctival coverage combined with amnion liquid supernatant eyedrop can effectively reduce keratitis cell infiltration of canine deep layer corneal ulcer, reduce corneal damage, and improve corneal transparency, the therapeutic effect is better than conjunctival coverage individually. Keywords : Canine, Alkali burn combined with mechanical injury, Amnion liquid supernatant, Conjunctival cover, Corneal ulcer