Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2024 , Vol 30 , Issue 4
Effects of Zinc Oxide Nanoparticles on Pyruvate Dehydrogenase and Lactate Dehydrogenase Expressions and Apoptotic Index in Breast Cancer Cells
Cem ÖZİÇ1, Barış YILDIZ2, Ramazan DEMİREL3, Özkan ÖZDEN4
1Kafkas University, School of Medicine Department of Medical Biology, TR-36100 Kars - TÜRKİYE
2Kafkas University, Institute of Health Sciences Department of Physiology, TR-36100 Kars - TÜRKİYE
3Kafkas University, Institute of Natural and Applied Sciences Department of Biotechnology, TR-36100 Kars - TÜRKİYE
4Kafkas University, Faculty of Engineering and Architecture Department of Bioengineering, TR-36100 Kars - TÜRKİYE
DOI : 10.9775/kvfd.2024.31688 Zinc oxide nanoparticles (ZnO-NPs) are metal oxide NPs that have high cytotoxicity on cancer cells and low cytotoxicity on healthy cells. Breast cancer is the most frequent type of cancer-causing death among women worldwide. In this study, anti-cancer effects of ZnO-NPs were investigated. For this purpose, we treated the MCF7 and MDA-MB-231 breast cancer cell lines and human umbilical vein endothelial cells HUVEC cell line with 10 μg/mL and 20 μg/mL ZnO-NP. Anti-cancer effects of ZnO-NPs were evaluated with cell viability, apoptotic index and colony formation assays, and anti-Warburg effect were investigated by evaluating of pyruvate dehydrogenase (PDH) and Lactate Dehydrogenase A (LDHA) protein expressions. Results indicated that, ZnO-NP application did not have a cytotoxic effect on HUVEC cells, it had cytotoxicity on both breast cancer cell lines. However, MCF7 cells were more sensitive to ZnO-NP treatment. Administration of 20 μg/mL ZnO-NP reduced the survival of MCF7 cells by 62% and increased the apoptotic index by approximately 6 times. Additionally, ZnO-NP treatment inhibited the doubling times of cells and suppressed the colony-forming abilities of both breast cancer cell lines. Also, it was seen that ZnO-NP treatment increased PDH expression in MCF7 cells, where the apoptotic index was more induced. As a result, we have shown for the first time that ZnO-NPs affect the energy metabolism of cells by increasing PDH expression in MCF7 cells, thus increasing the apoptotic index. Our study, which observed the anticancer effects of ZnO-NPs on breast cancer cells, will also shed light on future experimental studies. Keywords : Breast cancer, Lactate dehydrogenase A, MDA-MB-231, MCF7, Pyruvate dehydrogenase, Zinc oxide nanoparticles