Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2019 , Vol 25 , Issue 3
Establishment and Application of a Real-time, Duplex PCR Method for Simultaneous Detection of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis
Yuzi WU1, Hassan Z. A. ISHAG3, Lizhong HUA1, Lei ZHANG1, Beibei LIU1, Zhenzhen ZHANG1, Haiyan WANG1, Yanna WEI1, Zhixin FENG1, Hafizah Yousuf CHENIA2, Guoqing SHAO1, Qiyan XIONG1
1Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences· Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture· National Center for Engineering Research of Veterinary Bio- Products, Nanjing 210014 CHINA
2Discipline: Microbiology, School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, SOUTH AFRICA
3College of Veterinary Science, University of Nyala, Nyala, SUDAN
DOI : 10.9775/kvfd.2018.21137 The objective of this study was to develop a TaqMan probe-based, sensitive, specific duplex real-time PCR assay for simultaneous detection of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis. The specific primers and probes, labeled with FAM and Texas Red, respectively, were designed to amplify the p97 gene of M. hyopneumoniae and p37gene of M. hyorhinis. The duplex real-time PCR reaction mixtures were established and optimized and the sensitivity, specificity and reproducibility of the assay were assessed. The sensitivity of the duplex real-time PCR was found to be 10 copies/μL for both M. hyopneumoniae and M. hyorhinis, respectively. There was no cross reaction with other common viral and bacterial pathogens. The concentration of standard coefficient of variation of Ct values was less than 5%, indicating a good reproducibility. Clinical samples (n = 937) were tested by the duplex real-time PCR assay, including broncho-alveolar lavage fluids, nasal swabs, tissues and cell culture supernatant. Duplex real-time PCR for simultaneous detection of M. hyopneumoniae and M. hyorhinis was highly sensitive and can be utilized for diagnosing clinical samples. It is timeefficient and economic, thereby providing a new approach to control both M. hyopneumoniae and M. hyorhinis. Keywords : Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, duplex real-time PCR, Swine, Detection