Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2018 , Vol 24 , Issue 4
The Role of JAK2/STAT3 Signaling Pathway Regulation in Macrophage Apoptosis During Brucella M5-90 Infection
Jihai YI1, Yueli WANG1, Xiaoyu DENG1, Zhiran SHAO1, Huan ZHANG1, Benben WANG2, Junbo ZHANG3, Tiansen LI1, Chuangfu CHEN1
1College of Animal Science and Technology, Shihezi University, 832000-Shihezi City, Xinjiang, CHINA
2School of Life Science, Shihezi University, 832000-Shihezi City, Xinjiang, CHINA
3College of Biology, Agriculture and Forestry, Tongren University, 554300-Tongren, Guizhou, CHINA
DOI : 10.9775/kvfd.2018.19590 Brucellosis is serious zoonotic disease affecting both animals and humans. Brucella inhibits the apoptosis of host macrophages. And the JAK2/STAT3 pathway regulates various cellular physiological activities. However, the association between Brucella-mediated inhibition of macrophage apoptosis and regulation of the JAK2/STAT3 pathway is unclear. In the current study, We tested the the activation of JAK2/ STAT3 pathway and evaluated its function during Brucella M5-90 infection in cells. The result was found that infection with Brucella M5-90 activated the JAK2/STAT3 pathway and induced phosphorylation of both JAK2 and STAT3 in a time-dependent manner. JAK2 and STAT3 phosphorylation were inhibited by AG490 in a dose-dependent manner. Inhibition of the JAK2/STAT3 pathway with AG490 significantly induced proinflammatory responses, macrophage apoptosis at the transcriptional and protein levels, as well as intracellular survival and replication of Brucella M5-90. This study indicates that the JAK2/STAT3 pathway plays a major role in macrophage apoptosis induced by infection with Brucella M5-90. In addition, TNF-α plays a major role in the regulation of the JAK2/STAT6 pathway during Brucella M5-90 infection. The above information may help to unravel the pathogenic mechanism of Brucella infection. Keywords : Brucella, JAK2/STAT3 pathway, Apoptosis, Infection, AG490