¹College of Life Sciences, Shihezi University, 832003, Shihezi, Xinjiang, CHINA DOI : 10.9775/kvfd.2020.24679 This study aimed to investigate the inhibition effect of CD46 on the replication of bovine viral diarrhea viruses (BVDV). BVDV causes bovine viral diarrheal mucosal disease, and prevention of BVDV infection is very important for livestock production. Here, we have designed four pairs of effective siRNAs, and in vitro experiments showed that some of them can effectively inhibit the expression of BVDV receptor CD46. Further results showed that BVDV replication was efficiently inhibited and the yield decreased accordingly when expression of CD46 was decreased by siRNA. In conclusion, this study provides a new approach to defend against BVDV infection. Keywords : BVDV, CD46, Replication, RNAi