¹Department of Veterinary Medicine, College of Animal Science ＆ Technology, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, 832000, CHINA
²Department of Basic Medicine, School of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, 832002, CHINA
³Department of Parasitology and Zoology, University of Veterinary Medicine, 1112, Budapest, HUNGARY
⁴Department of Forestry, College of Agriculture, Shihezi University, Shihezi, Xinjiang, Uygur Autonomous Region, 832000, CHINA DOI : 10.9775/kvfd.2020.24080 Twenty-five dog fleas (Ctenocephalides canis) and five ticks identified as Hyalomma asiaticum were collected from a Eurasian lynx (Lynx lynx) pup in northwestern China. Molecular analyses of four genetic markers showed the presence of Rickettsia aeschlimannii DNA in 5 out of 20 fleas. Only rickettsial 17-kDa gene was detected in the blood sample of the lynx. In addition, 2 out of 20 fleas were positive to Wolbachia endosymbiont by targeting 16S rDNA while there was no Bartonella DNA found both in 5 ticks and 20 fleas by using gltA and 16S-23S ITS. Our findings suggest that i) C. canis parasitizing wild Eurasian lynx harbors R. aeschlimannii and Wolbachia endosymbiont in the China-Kazakhstan border, and ii) Wolbachia endosymbiont in present study is closer to that in C. canis infesting sheltered dogs in Turkey. Keywords : Eurasian lynx, Ctenocephalides canis, Hyalomma asiaticum, Rickettsia aeschlimannii, Wolbachia endosymbiont