¹Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences· Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture· National Center for Engineering Research of Veterinary Bio- Products, Nanjing 210014 CHINA
²Discipline: Microbiology, School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, SOUTH AFRICA
³College of Veterinary Science, University of Nyala, Nyala, SUDAN DOI : 10.9775/kvfd.2018.21137 The objective of this study was to develop a TaqMan probe-based, sensitive, specific duplex real-time PCR assay for simultaneous detection of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis. The specific primers and probes, labeled with FAM and Texas Red, respectively, were designed to amplify the p97 gene of M. hyopneumoniae and p37gene of M. hyorhinis. The duplex real-time PCR reaction mixtures were established and optimized and the sensitivity, specificity and reproducibility of the assay were assessed. The sensitivity of the duplex real-time PCR was found to be 10 copies/μL for both M. hyopneumoniae and M. hyorhinis, respectively. There was no cross reaction with other common viral and bacterial pathogens. The concentration of standard coefficient of variation of Ct values was less than 5%, indicating a good reproducibility. Clinical samples (n = 937) were tested by the duplex real-time PCR assay, including broncho-alveolar lavage fluids, nasal swabs, tissues and cell culture supernatant. Duplex real-time PCR for simultaneous detection of M. hyopneumoniae and M. hyorhinis was highly sensitive and can be utilized for diagnosing clinical samples. It is timeefficient and economic, thereby providing a new approach to control both M. hyopneumoniae and M. hyorhinis. Keywords : Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, duplex real-time PCR, Swine, Detection