Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2018 , Vol 24 , Issue 6
Y-Chromosome Polymorphisms in 12 Native, Karagül, Karacabey Merino Breeds from Turkey and Anatolian Mouflon (Ovis gmelinii anatolica)
Arif PARMAKSIZ1, Ahmet OYMAK1, Eren YÜNCÜ2, Sevgin DEMİRCİ2, Evren KOBAN BAŞTANLAR3, Emel ÖZKAN ÜNAL4, İnci TOGAN2, Füsun ÖZER2
1The Department of Biology, Faculty of Science-Literature, Harran University, TR-63290 Şanlıurfa - TURKEY
2The Department of Biological Sciences, Middle East Technical University, TR-06800 Ankara - TURKEY
3The Department of Biology, Faculty of Science, Ege University, TR-35100 İzmir - TURKEY
4The Department of Animal Science, Faculty of Agriculture, Namık Kemal University, TR-59030 Tekirdağ - TURKEY DOI : 10.9775/kvfd.2018.19962 In this study, 182 male animals from 12 native sheep breeds, as well as Karacabey Merino and Karagül breeds of Anatolia, wild sheep Anatolian Mouflon (Ovis gmelinii anatolica) were used as the study material. Based on SRY and SRYM18 regions on the Y-chromosome, haplotypes of the populations were analyzed using DNA sequence analyses. The SRY region, A-oY1 allele was observed in all of the individuals studied. On the other hand, four different alleles corresponding to four Y-chromosome haplotypes were detected at the SRYM18 microsatellite region. Among native Anatolian breeds (n=143), H6 haplotype (80.41%), H4 haplotype (9.09%), H8 haplotype (8.40%) and H12 haplotype (2.1%) were identified. H6 haplotype was observed in all 16 individuals of Ovis gmelinii anatolica. Pairwise FST values based on haplotype frequencies were calculated for domestic sheep, and the highest FST value was observed between Karagül and Kıvırcık along with Karagül and Ovis gmelinii anatolica with pairwise FST value of 0.43202 (P<0.01). Y chromosome polymorphism of sheep from Turkey were examined comparatively with the accumulated data in the literature. Out of seven haplotypes (H4, H5, H6, H7, H8, H12, H19) observed in Europe and Asia, 4 haplotypes (H4, H6, H8 and H12) were observed in Anatolia. H12 was a private haplotype of Sakız, H6 seems to be the predominant haplotype of domestic sheep (79.51%) as well as being the only haplotype observed in Ovis gmelini anatolica. H4 haplotype seemed to be associated with fat tailed sheep migrating to Turkey, entering from south east of Turkey, which may be related with the arrival of nomadic Turks. Keywords : Y-Chromosome haplotypes, SRY, SRYM18, Ovis gmelinii anatolica, Native sheep breeds of Turkey