¹Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, CHINA
²Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, CHINA
³Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637002, CHINA DOI : 10.9775/kvfd.2016.17329 The purpose of this study was to investigate the effects of chitosan oligosaccharides (COS) on the antioxidative function, lymphocyte cycle and apoptosis of ileum mucosa in broiler. 640 AA broilers were randomly allocated into four groups, which were fed with diets supplemented 0, 200, 350 and 500 mg/kg of COS for six weeks, respectively. The results showed that compared with the control group, the activities of glutathione peroxidase and superoxide dismutase, the ability of total antioxidant capacity and inhibit hydroxy radical as well as the contents of glutathione in the 350 and 500 mg/kg COS groups were significantly increased, while the levels of malonedialdehyde were significantly decreased. The percentages of S and gap 2/mitosis (G2M) phases and proliferating index of ileum mucosal lymphocytes in the 350 and 500 mg/kg COS groups were increased, but, the percentages of apoptotic ileac lymphocytes were not significantly different compared with the control group. No significant difference in the levels of antioxidant function mentioned above, cell cycle phase distribution and the percentages of apoptotic ileac lymphocyte existed between the 350 and 500 mg/kg COS groups. Conclusion: dietary chitosan oligosaccharides supplements with 350 mg/kg and 500 mg/kg could improve the antioxidant function and accelerate lymphocytes proliferation, but had non-influence on lymphocytes apoptosis in the ileum mucosa of broiler Keywords : Chitosan oligosaccharides, Lymphocyte, Antioxidant function, Cell cycle, Apoptosis