¹Erciyes University, Faculty of Veterinary Medicine, Department of Genetics, TR-38039 Kayseri - TURKEY
²Ankara University, Faculty of Veterinary Medicine, Department of Biostatistics, TR-06110 Ankara - TURKEY
³Erciyes University, Faculty of Veterinary Medicine, Department of Biometrics, TR-38039 Kayseri - TURKEY DOI : 10.9775/kvfd.2021.25714 This study aims to investigate the eff ects of three single nucleotide (SNPs) polymorphisms of the MBL1 gene on subclinical mastitis for Holstein cows. For this study, a total of 151 Holstein cows were selected in their third lactation. The subclinical mastitis conditions were determined using the California Mastitis Test (CMT). Genotyping was carried out with the PCR-RFLP method. The results revealed that the wild-type allele frequencies were 0.72 (G allele), 0.40 (G allele), and 0.37 (T allele) for 1252 G>A, 2534 G>A, and 2569 T>C SNPs, respectively. Among the investigated SNPs, only the 1252 G>A SNP was not found in Hardy-Weinberg equilibrium. The eff ects of SNPs on subclinical mastitis were modeled using Multiple Logistic Regression Analysis. The established model can identify cows with subclinical mastitis with a separation efficiency of 62.3%. It was thought that 1252 G> A and 2534 G> A SNPs may aff ect subclinical mastitis rates. Keywords : Cow, MBL1, Multiple logistic regression analysis, SNP, Subclinical mastitis