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Kafkas Üniversitesi Veteriner Fakültesi Dergisi
2020 , Vol 26 , Issue 1
Development of a Monoclonal Antibody to Detect αs1-casein in the Milk of Healthy and Mastitis-Affected Goats
1Veterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINA2China Animal Health and Epidemiology Center, Qingdao 266032, Shandong Province, CHINA DOI : 10.9775/kvfd.2019.22287 This study aimed to evaluate the expression level of caseins during mastitis of goats. Whole goat caseins were used as primary antigens for mouse immunization. A monoclonal antibody (mAb) named 5B with high specificity to goat αs1-casein was developed. Further results showed that mAb 5B can successfully be applied to western blot and ELISA. In addition, immunofluorescence analysis using this mAb showed increased milk αs1-casein level in mastitis-affected goats. In conclusion, this study has established an effective tool to evaluate the expression level of αs1-casein during mastitis development. Keywords : αs1-casein, Monoclonal antibody, ELISA, Mastitis, Immunofluorescence, Goat