¹Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-15000 Burdur - TURKEY
²Ankara University Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Şehit Ömer Halisdemir Avenue, TR-06110 Ankara - TURKEY
³Ankara University Department of Biostatistics, Faculty of Veterinary Medicine, Şehit Ömer Halisdemir Avenue, TR-06110 Ankara - TURKEY DOI : 10.9775/kvfd.2018.19958 The aim of this study was to evaluate the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol loaded cyclodextrin (CLC) with the addition of different glycerol (G) rates on bull semen cryopreservation. Semen samples of three bulls were pooled and divided into seven groups; control group (C) and the other six were extended with standard Tris extender including different G rates (3, 6 and 9%) with CLC or 7-DHCLC to reach 1.5 mg/120x106. Extended samples were frozen in 0.25 mL straws. After thawing, motion characteristics and motility, viability, acrosome integrity, mitochondrial activation, functional membrane integrity and abnormal spermatozoa rates were assessed. The highest membrane integrity, viability and total motility were detected in 7-DHCLC with 9% of G group (P<0.001). The lowest progressive motility was detected in 3% G groups (P<0.001). 7-DHCLC with 9% G had higher mitochondrial activity compared with 3% G groups. In addition, no statistical difference was observed between groups in terms of acrosome integrity and abnormal spermatozoa rates (P>0.05). According to these results, 7-DHCLC with 9% G addition maintained some spermatological parameters better than other groups after thawing in bull semen thus, it was concluded that 7-DHCLC could be used instead of CLC in bull semen cryopreservation. Keywords : Cholesterol 7-dehydrocholesterol, Cyclodextrin, Glycerol, Bull semen, Freeze-thawed