Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2017 , Vol 23 , Issue 6
Effect of Commercial Toxin Binder, Native Probiotic Strains, Cell Wall Yeast and Aluminosilicate in Diets Contaminated with Aflatoxin, on the Expression of GOT2, CYP450 1A5 Genes and Serum Concentrations of Liver Enzymes in Broiler Chickens
Mohsen BARATI1, Mohammad CHAMANI1, Seyed Naser MOUSAVI2, Seyed Abdollah HOSEINI3, Maryam Taj Abadi EBRAHIMI4
1Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, IRAN
2Department of Animal Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Tehran, IRAN
3Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization, Karaj, IRAN
4Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, IRAN DOI : 10.9775/kvfd.2017.18022 This study was conducted to investigate the effect of cell wall yeast, aluminosilicate and some probiotic strains in diets contaminated with aflatoxin, on the expression of GOT2, CYP450 1A5 genes and serum concentrations of liver enzymes including GGT, ALT, AST and LDH in broilers. 400 seven-days old chicks from strain Cobb 500 were used as a completely randomized design with 8 treatments, 5 replications. Treatments were included: 1- Negative control (NC), 2- NC + 1 mg/kg aflatoxin or positive control (PC), 3- PC + 1 kg/ton Toxeat® (a toxin binder), 4- PC + Lactobacillus strains (L), 5- PC + Bacillus subtilis JQ618 strain (B), 6- PC + Saccharomyces cerevisiaeʼs cell wall (Y), 7- PC + [PC+ BLY ) B+ L+ Y(], 8- PC + Hydrated sodium calcium Aluminosilicate (HA). The serum concentrations of LDH, ALT, GGT and AST were increased in PC group at 42d (P˂0.05). Tox®, L and BLY reduced serum levels of AST (P˂0.05). Increased serum concentration of GGT was observed in PC treatment, decreased by HA, Y, B and L treatments (P˂0.05). The results showed the upregulation of GOT2, CYP450 1A5 in PC group. But Y, B, L and Tox® reduced the expression of GOT2. The groups receiving aflatoxin adsorbent compounds reduced the adverse effects of aflatoxin on increasing the expression of CYP450 1A5. Keywords : Aflatoxin, Broiler Chickens, Gene Expression, Liver Enzymes, Probiotic strains, Toxeat®