¹Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, CHINA DOI : 10.9775/kvfd.2016.16371 Myogenin gene encodes for skeletal muscle-specific transcription factors with highly conserved basic helix-loop-helix domain and play key role in growth and muscle development. In this study, the polymorphisms of myogenin gene were investigated to check whether they are associated with growth traits in Chinese-Tibetan sheep. In three sheep breeds with a total number of 632 individuals, three SNPs, including two in exon 1 (C109A and C183T) and one in intron 2 (A1403C) were found by DNA sequencing. Among those, C109A is a missense mutation (Gly37Arg). The single marker association analysis showed that the three mutations were significantly associated with growth traits (at P<0.01 or P<0.05). In conclusion, our results suggest that myogenin gene variation may be considered molecular markers for growth traits in Chinese-Tibetansheep. Keywords : Chinese Tibetan sheep, Myogenin gene, SNP, Growth traits