Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2024 , Vol 30 , Issue 2
Complete Mitochondrial Genome Analyses of Forcipomyia pulchrithorax (Diptera: Ceratopogonidae): Genome Orientation and Phylogenetic Implications
Gupse Kubra KARADEMIR1, Saffet TEBER2, Canan CANER KULIG1, Ahsen Meliha TOROSLU1, Osman IBIS2, Alparslan YILDIRIM1
1Erciyes University, Faculty of Veterinary Medicine, Department of Parasitology, TR-38039 Kayseri - TÜRKİYE
2Erciyes University, Faculty of Agriculture, Department of Agricultural Biotechnology, TR-38039 Kayseri - TÜ
DOI : 10.9775/kvfd.2023.30825 Forcipomyia Meigen, 1818, is the second-largest genus of the family Ceratopogonidae, having medico veterinary and agricultural importance. A few mitogenomes of Ceratopogonidae have previously been available. Here, we aimed to characterize the whole mitogenome of Forcipomyia pulchrithorax Edwards, 1924, collected from Central Anatolia Region of the Türkiye using NGS to contribute to the genetic diversity within this family. The mitogenome of F. pulchrithorax (OR666457) consisted of a circular DNA molecule spanning 15,930 bp and containing 72.7% AT content. It includes 13 proteincoding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 1 control region. All PCGs exhibited typical ATN start codons and followed the conventional TAN stop codons, except COX1, COX2, COX3, ND1, and ND5, which ended with incomplete codons. The 22 tRNA genes demonstrated the ability to form a cloverleaf structure, except tRNA-Ser1, which lacked DHU arm, similar to most insect mitogenomes. The ML phylogenetic analyses clearly delineated the species of Culicomorpha into well supported monophyletic clades. Forcipomyia pulchrithorax was clustered with the Ceratopogonidae species constituting an outer taxon among the Culicomorpha. The comprehensive mitogenome of F. pulchrithorax will be valuable for future studies focused on the phylogenetic characterization and diversity of Ceratopogonidae within the Culicomorpha. Keywords : Culicomorpha, Diptera, Forcipomyia pulchrithorax, Mitochondrial genome, Phylogeny