Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Determination of Macro and Trace Element Levels of Serum, Tears, Saliva, and Hair Samples in Kilis Goats with ICP-MS
Nilgün PAKSOY1, Mehmet İRİADAM2, Faruk BOZKAYA3, Ünal YAVUZ4, Egemen Erdem ÖZTÜRK1
1Department of Biochemistry, Faculty of Veterinary Medicine, Harran University, TR-63200 Şanlıurfa - TÜRKİYE
2Department of Physiology, Faculty of Veterinary Medicine, Harran University, TR-63200 Şanlıurfa - TÜRKİYE
3Department of Genetic, Faculty of Veterinary Medicine, Harran University, TR-63200 Şanlıurfa - TÜRKİYE
4Department of Surgery, Faculty of Veterinary Medicine, Harran University, TR-63200 Şanlıurfa - TÜRKİYE
DOI : 10.9775/kvfd.2022.27674 This study is focused on the evaluation of macro and trace elements concentration including sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), copper (Cu), zinc (Zn), selenium (Se), nickel (Ni), titanium (Ti), manganese (Mn), cobalt (Co), and chromium (Cr) of the serum, saliva, tears, and hair samples in Kilis goats. The study involved 33 goats without clinical signs of disease. Individual serum, saliva, tears, and hair samples from each goat were collected for element quantifications through the inductively coupled plasma mass spectrometry (ICP-MS) method after acid digestion in a microwave system. Element concentrations varied and depended on the sample type. The most predominant element in serum and saliva samples was Na (3559±55.2 and 3265±53.6 mg/L, respectively), followed by K>Ca>Mg. Potassium was the most abundant element analyzed in tears and hair samples (3506±305 and 4664±100.5 mg/L, respectively). The major trace element was Fe in all sample types except hair samples. Nickel was detected only in serum samples (83.9±6.35 μg/L) and Mn was detected only in hair samples (12903±3142 μg/L). In all samples, Co was below the detection limit. There was a significant correlation of some elements between serum and hair (Na, K, Mg, Cu), tears and hair (K, Mg, Fe), serum and tears (K) samples. Trace elements concentration did not pose a risk for deficiency or excess. It was concluded that tears and saliva are potential alternative materials for element analysis and simultaneous tears, saliva, and hair along with serum could be useful in predicting the inorganic metabolic status of goats. Keywords : Element, Goat, Hair, ICP-MS, Saliva, Tears