Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2022 , Vol 28 , Issue 2
Hydrogen Relieves Neuropathic Pain in Diabetic Rats by Inhibiting MCP1 and CCR2 Expressions
Pin WANG1, Lanying YOU2, Qian LI3, Hao WANG3, Yun LONG3, Wei CHEN4
1Department of Anesthesiology, Nanjing Yimin Hospital, Shanggao Road 86, Dongshan Street, Nanjing 210000, Jiangsu Province, P. R. CHINA
2Department of Anesthesiology, Nanjing Pukou Hospital of TCM, Gongyuan North Road 18, Nanjing 210000, Jiangsu Province, P. R. CHINA
3Department of Anesthesiology, Affi liated Jiangning Hospital of Nanjing Medical University, Hushan Road 169, Nanjing 211100, Jiangsu Province, P. R. CHINA
4Department of Otolaryngology-Head and Neck, Jinling Hospital, School of Medicine; Nanjing University, Nanjing 210046, Jiangsu Province, P. R. CHINA
DOI : 10.9775/kvfd.2021.26628 We aimed to explore the mechanism for hydrogen in the treatment of neuropathic pain in diabetic rats. Eight-week-old male SD rats were randomly divided into control, model and hydrogen treatment groups. Th e hydrogen treatment group was intraperitoneally injected with hydrogen-rich saline daily in 7th and 8th weeks aft er modeling. Before and 2, 4, 6 and 8 weeks aft er modeling, the neurological function, behavioral changes and levels of infl ammatory factors TNF-α and IL-6 in the sciatic nerve were detected, and MCP1 and CCR2 protein expressions were measured by Western blotting. Compared with the model group, the hydrogen treatment group had signifi cantly increased motor nerve conduction velocity, thermal withdrawal latency and mechanical withdrawal threshold (P<0.05). Th e signifi cantly higher levels of TNF-α and IL-6 in the sciatic nerve of the model group than those of the control group decreased in the hydrogen treatment group (P<0.05). Th e protein expressions of MCP1 and CCR2 in the sciatic nerve of the model group signifi cantly exceeded those of the control group. Such expressions of the hydrogen treatment group were lower than those of the model group. Hydrogen alleviated the inflammatory response of peripheral nerves in diabetic rats by inhibiting the MCP1-CCR2 signaling pathway, thus mitigating neuropathic pain. Keywords : Diabetic neuropathy, Hydrogen, MCP1, CCR2, TNF-α, IL-6