Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
Effect of Imidocarb on DNA Damage in Sheep with Babesiosis
Ahmet Cihat ÖNER1, Adnan AYAN2, Özlem ORUNÇ KILINÇ3, Ayşe USTA4, Fatma ERTAŞ5
1Van Yüzüncü Yıl University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, TR-65080 Van - TÜRKİYE
2Van Yüzüncü Yıl University, Faculty of Veterinary Medicine, Department of Genetics, TR-65080 Van - TÜRKİYE
3Van Yüzüncü Yıl University Özalp Vocational School of Higher Education, Department of Medical Laboratory Technician, TR-65800 Van - TÜRKİYE
4Van Yüzüncü Yıl University, Faculty of Science, Department of Chemistry, TR-65080 Van - TÜRKİYE
5Igdır University, Tuzluca Vocational School of Higher Education, TR-76000 Iğdır - TÜRKİYE
DOI : 10.9775/kvfd.2021.26607 In this study, it was aimed to determine the DNA damage using the comet assay, which specifically shows DNA damage in naturally Babesia spp.-infected sheep and to evaluate the damage before and after imidocarb application. Blood samples obtained from 10 infected sheep with positive clinical signs and symptoms of babesiosis and whose diagnosis was confirmed by Giemsa staining and PCR methods, and blood samples from 10 healthy sheep were used as study material. DNA damage was examined by the comet assay from the blood samples of the infected patient group and the control group obtained during the disease and after the treatment, and the results were compared with statistical methods. When DNA damage was examined in sick animals diagnosed with babesiosis, the tail length and the tail moment values were found to be statistically significantly higher than the control group (P<0.0001). According to the results obtained after imidocarb application, it was determined that DNA damage and tail moment decreased statistically with imidocarb, and the difference was statistically significant, and the values were higher than the control group (P<0.0001). As a result, Babesia infection can cause DNA damage, has been confirmed by the determination of direct DNA damage using the comet assay, and imidocarb given for treatment was successful and reduced the damage. Keywords : Babesiosis, DNA damage, Imidocarb, Sheep