Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 4
Activity of Disinfecting Biocides and Enzymes of Proteases and Amylases on Bacteria in Biofilms
Mykola KUKHTYN1, Vladislav KOZHYN2, Victor HORIUK2, Zoya MALIMON3, Yulia HORIUK4, Tetyana YASHCHUK5, Sergiy KERNYCHNYI2
1Ternopil Ivan Pului National Technical University, Faculty of Engineering of Machines, Structures and Technologies, Department of Food Biotechnology and Chemistry, 46001, UKRAINE
2State Agrarian and Engineering University in Podilya, Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Pathology and Surgery, 32300, UKRAINE
3State Research Institute for Laboratory Diagnostics and Veterinary and Sanitary Expertise, 02000, UKRAINE
4State Agrarian and Engineering University in Podilya, Faculty of Veterinary Medicine and Technologies in Livestock, Department of Infectious and Parasitic Diseases, 32300, UKRAINE
5Ternopil State Agricultural Experimental Station of Institute of feed and agriculture of Podillya of NAAS, 46027, UKRAINE
DOI : 10.9775/kvfd.2021.25770 The presence of microbial biofilms on the surfaces of medical instruments, operating equipment, prostheses, catheters, technological lines in the food industry is a fact that contributes to the infection of the macroorganism and contamination of raw materials and products. The aim of the work was to investigate the eff ect of disinfecting substances Vantocilu TG and Catamine AB and their combination with enzymes on bacteria in biofilms. In the experiments, we used disinfecting substances Vantocil TG (Arch Biocides LTD, Great Britain) and Catamine AB (Intersintez, Ukraine). Enzymes: Everlase 16 L and Termamyl 300 L (Novozymes, Denmark). It was found that bacteria in biofilms withstood the minimum bactericidal concentration of Vantocil and Catamine, which was set on their planktonic forms. From one mL of wash from the biofilm after exposure to Vantocil were isolated from 1.9×103 to 4.3×103 microbial cells, and after treatment with Catamine from 5.6×103 to 1.7×104. At the same time, after treatment of biofilms with Vantocil and Catamine together with enzymes, a decrease in the number of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa cells was observed, on average by two orders up to 101 CFU/mL, compared with treatment with biocides only. That is, there is a clear synergy of enzymes and biocides, which ultimately has a more detrimental eff ect on bacteria in biofilms. Keywords : Vantocil TG, Catamine AB, Enzymes, Biofi lm degradation