Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 3
The Effects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows
Esma Gamze AKSEL1, Aytaç AKÇAY 2, Korhan ARSLAN1, Mahmodul Hasan SOHEL1, Güven GÜNGÖR3, Bilal AKYÜZ1
1Erciyes University, Faculty of Veterinary Medicine, Department of Genetics, TR-38039 Kayseri - TURKEY
2Ankara University, Faculty of Veterinary Medicine, Department of Biostatistics, TR-06110 Ankara - TURKEY
3Erciyes University, Faculty of Veterinary Medicine, Department of Biometrics, TR-38039 Kayseri - TURKEY
DOI : 10.9775/kvfd.2021.25714 This study aims to investigate the eff ects of three single nucleotide (SNPs) polymorphisms of the MBL1 gene on subclinical mastitis for Holstein cows. For this study, a total of 151 Holstein cows were selected in their third lactation. The subclinical mastitis conditions were determined using the California Mastitis Test (CMT). Genotyping was carried out with the PCR-RFLP method. The results revealed that the wild-type allele frequencies were 0.72 (G allele), 0.40 (G allele), and 0.37 (T allele) for 1252 G>A, 2534 G>A, and 2569 T>C SNPs, respectively. Among the investigated SNPs, only the 1252 G>A SNP was not found in Hardy-Weinberg equilibrium. The eff ects of SNPs on subclinical mastitis were modeled using Multiple Logistic Regression Analysis. The established model can identify cows with subclinical mastitis with a separation efficiency of 62.3%. It was thought that 1252 G> A and 2534 G> A SNPs may aff ect subclinical mastitis rates. Keywords : Cow, MBL1, Multiple logistic regression analysis, SNP, Subclinical mastitis