Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 3
Identification of a New MLST Subtype of Cryptosporidium muris from a Brown Rat (Rattus norvegicus) in China
Xuehan LIU1, Lei DENG2, Zhijun ZHONG2, Ziyao ZHOU2, Xuefeng YANG1, Zhixing AN1
1College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, CHINA
2College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 625014, CHINA
DOI : 10.9775/kvfd.2021.25681 Cryptosporidium muris is a zoonotic protozoan. Characterization of genetic diff erentiation can be exploited to trace the origins of the parasite. A Cryptosporidium isolate from brown rat was obtained by microscopy and genetically characterized. C. muris was identified with minor nucleotide diff erences. Further subtype analysis was performed using a multilocus sequence typing (MLST) tool. At each locus, the C. muris isolate was confirmed to be M12, M4, M1 and M2 subtype, representing as a new MLST subtype. Therefore, the results suggest that there was probably a new source of infection for C. muris in the study region. Keywords : Cryptosporidium muris, 18S rRNA, MLST, Subtype, Brown rat