Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2020 , Vol 26 , Issue 1
Development of a Monoclonal Antibody to Detect αs1-casein in the Milk of Healthy and Mastitis-Affected Goats
Ming PANG1, Xiong GUAN1, Chen-Xiang ZUO1, Ming-Jie LIU1, Saad REHMAN1, Qin-Lei FAN2, Ping LU2, De-Kun CHEN1, Wen-Tao MA1
1Veterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINA
2China Animal Health and Epidemiology Center, Qingdao 266032, Shandong Province, CHINA
DOI : 10.9775/kvfd.2019.22287 This study aimed to evaluate the expression level of caseins during mastitis of goats. Whole goat caseins were used as primary antigens for mouse immunization. A monoclonal antibody (mAb) named 5B with high specificity to goat αs1-casein was developed. Further results showed that mAb 5B can successfully be applied to western blot and ELISA. In addition, immunofluorescence analysis using this mAb showed increased milk αs1-casein level in mastitis-affected goats. In conclusion, this study has established an effective tool to evaluate the expression level of αs1-casein during mastitis development. Keywords : αs1-casein, Monoclonal antibody, ELISA, Mastitis, Immunofluorescence, Goat