Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2019 , Vol 25 , Issue 1
DGAT1, CAST and IGF-I Gene Polymorphisms in Akkaraman Lambs and Their Effects on Live Weights up to Weaning Age
Davut BAYRAM1, Bilal AKYÜZ2, Korhan ARSLAN2, Fadime ÖZDEMİR2, Esma Gamze AKSEL2, Mehmet Ulaş ÇINAR3
1Erciyes Üniversitesi, Department of Animal Breeding and Husbandry, TR-38280 Kayseri - TURKEY
2Erciyes Üniversitesi, Department of Genetics, Faculty of Veterinary Medicine, TR-38280 Kayseri - TURKEY
3Erciyes Üniversitesi, Department of Animal Science, Faculty of Agriculture, TR-38280 Kayseri - TURKEY
DOI : 10.9775/kvfd.2018.20055 Early live weight in sheep is important for lamb survival and average weight gain until slaughter. The aim of the present study was to investigate association between CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms and early live weights between birth and weaning age in Akkaraman lambs. A total of 374 lambs were genotyped for CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms by the polymerase chain reaction and restriction length polymorphism (PCR-RFLP) method. The results of PCR-RFLP analysis showed that the SNPs had three genotypes of CAST-MspI polymorphism, two genotypes of DGAT1-AluI polymorphism and one genotype of IGF-1-Bsp143II polymorphism of these, CAST-MspI-MM, DGAT1-AluI-CC and IGF-1-Bsp143II-BB were the predominant genotypes in the Akkaraman sheep breed. The result of Chi-square analysis indicated that the Akkaraman sheep breed was in Hardy-Weinberg equilibrium for the investigated polymorphic genes. At the DGAT1 locus, the CT genotype showed significantly heavier birth weight (P=0.044) compared to CC genotype. CAST gene did not show any association for the investigated traits. The results of this study demonstrate that the CT genotype had a positive effect on birth weight in Akkaraman sheep. We concluded that further investigations are needed in DGAT1-AluI polymorphism and live weight at different ages in sheep. Keywords : Birth weight, DGAT1, polymorphism, RFLP, Sheep