¹Henan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Veterinary Medicine Department, 453003, Xinxiang City, CHINA
²Henan International Joint Laboratory of Animal Health Breeding and Disease Prevention and Control, Henan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Veterinary Medicine Department, 453003, Xinxiang City, CHINA DOI : 10.9775/kvfd.2026.36538 This study investigated the antibacterial activity and disinfection efficacy of chlorocresol nanoemulsion disinfectant (CND) against Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined, and alterations in bacterial ultrastructure were examined using scanning and transmission electron microscopy (SEM and TEM). The disinfection performance was further evaluated through simulated and field surface disinfection trials. The results showed that the MIC and MBC of CND against E. coli were 100 μg/mL and 200 μg/mL, respectively. After 0.06% CND treatment for 10 min, SEM revealed disrupted surface architecture with flagella loss and cell wall damage, while TEM showed compromised internal integrity including periplasmic narrowing and cytoplasmic aggregation. The disinfectant efficacy of CND increased with increasing contact time and concentration. In simulated surface trials, effective disinfection was achieved with 0.1% CND applied for ≥15 min or with CND concentrations ≥0.2%. In field trials, effective disinfection was achieved with 0.05% CND applied for ≥10 min or with CND concentrations ≥0.1%. These findings indicate that CND exhibits strong antibacterial activity against E. coli, causes significant ultrastructural alterations, and demonstrates effective disinfection performance. Keywords : Antibacterial activity, Chlorocresol nanoemulsion disinfectant, Disinfection efficacy, E. coli, Ultrastructure