¹Universitas Padjadjaran, Faculty of Animal Husbandry, Department of Animal Nutrition and Feed Technology, 45363 Bandung, INDONESIA
²Universitas Padjadjaran, Faculty of Animal Husbandry, Animal Husbandry Program PSDKU Pangandaran, 46393 Pangandaran, INDONESIA DOI : 10.9775/kvfd.2025.33784 An experiment was conducted to evaluate the effect of various culture media used to provide nutrients for lactic acid bacteria (LAB) on performance, carcass yield, blood parameters, natural antibody titers, and lipid and protein metabolism namely plasma triglycerides, total protein, albumin, and urea in broiler chickens. A total of 400 one-dayold Cobb broiler chicks was allocated to four treatments: a control feed (no probiotics), feed supplemented with 2% probiotic powder, which had been cultured in one of the following media: (1) 100% cow milk (CM), (2) a mixture of 50% cow milk and 50% soybean milk (SM), or (3) a combination of 50% cow milk, 25% soybean milk, and 25% mung bean milk (MM), reared for 28 days of experimental period. The results showed that, birds fed SM exhibited higher body weight, body weight gain, feed intake, and better feed conversion ratio than other groups, as well as higher carcass percentages, lower abdominal fat, and lower plasma and meat cholesterol. They also showed significantly lower plasma triglyceride, total protein, and albumin levels (P<0.01) and reduced IgY and IgM antibody titers binding keyhole limpet hemocyanin (KLH). Nonetheless, all treatments demonstrated an increasing immune response over time. These data indicate that a 50:50 cow milk and soybean milk probiotic culture could enhance broiler performance, carcass yield, plasma lipid metabolism, blood metabolite profiles (such as protein, albumin, and urea) and immune status. Keywords : Antibody titers, Broiler, Carcass yield, Immunoglobulin Y, Lipid metabolism, Probiotic