¹Department of Parasitology, Faculty of Veterinary Sciences, University of Agriculture, 38000, Faisalabad, PAKISTAN
²Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Sharif University of Agriculture, 66000, Multan, PAKISTAN
³Department of Animal Science, Faculty of Animal and Agricultural Science, Universitas Diponegoro, Semarang, 50275, Central Java, Indonesia
⁴Faculty of Veterinary and Animal Sciences, The Islamia University Bahawalpur, 63100, Bahawalpur, PAKISTAN
⁵International Joint Laboratory for One Health and Food Safety, College of Veterinary Medicine of Zhejiang A&F University, 666 Wusu Street, Lin’an District, Hangzhou, 311300, Zhejiang, China
⁶Department of Clinical Sciences, University of Veterinary and Animal Sciences Sub-campus Jhang, 35200, Jhang, PAKISTAN
⁷Department of Zoology, College of Science, King Saud University, 1145, Riyadh, SAUDI ARABIA DOI : 10.9775/kvfd.2025.33594 In present study the anticoccidial activity of essential oil of Trachyspermum ammi (seeds) was evaluated by in vivo methods. For in vivo trial, total of 72 (day-old) broiler chicks were randomly divided into six groups A, B, C, D, E and F (each group having 12 chicks). At one week of age groups A, B, and C were treated with essential oil of Trachyspermum ammi (seeds) at concentration 1%, 2%, and 3% by supplementing in feed for three consecutive days. Group D served as positive control (infected and medicated with Toltrazuril treatment), group E served as negative control group (infected and nonmedicated) and group F as normal (non-infected and non-medicated) control group. All groups except F were infected orally with 50.000 sporulated oocysts of mixed Eimeria species. Anticoccidial potential of essential oil was determined by different parameters such as mortality rate, fecal score, oocyst per gram of feces, weight gain, feed conversion ratio. Furthermore, hematological and serum chemistry profile was also evaluated. Hence treatment of Trachyspermum ammi (seeds) essential oil at dose rate 1%, 2% and 3% supplementation produces anticoccidial effects in terms of enhanced feed conversion ratio, decreased fecal and OPG count and improved weight gain in infected chickens. These treatment of essential oil showed to put positive effects also on hematological parameters like hemoglobin level, packed cell volume, red blood cells and white blood cells count and also put positive response towards serum biochemistry. Keywords : Trachyspermum ammi, Essential oil, Coccidiosis, Chicken