Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2025 , Vol 31 , Issue 2
In Vitro Effect of Boron Compounds in Combination with Photobiomodulation Therapy by 905 nm on the Viability of Human Gingival Fibroblasts
Ferda TURGUT1, Latif Emrah YANMAZ2, Serkan YILDIRIM3, Ali TAGHIZADEHGHALEHJOUGHI4, Yesim YENI5, Sitkican OKUR6, Omer Tarik ORHUN7
1Department of Surgery, Faculty of Veterinary Medicine, Cukurova University, TR-01130 Adana - TÜRKİYE
2Department of Surgery, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, TR-15030 Burdur - TÜRKİYE
3Department of Patology, Faculty of Veterinary Medicine, Ataturk University, TR-25240 Erzurum - TÜRKİYE
4Department of Medical Pharmacology, Faculty of Medicine, Seyh Edebali University, TR-11000 Bilecik - TÜRKİYE
5Department of Medical Pharmacology, Faculty of Medicine, Turgut Ozal University, TR-44210 Malatya - TÜRKİYE
6Department of Surgery, Faculty of Veterinary Medicine, Ataturk University, TR-25240 Erzurum - TÜRKİYE
7Department of Surgery, Faculty of Veterinary Medicine, Necmettin Erbakan University, TR- 42310 Konya - TÜRKİYE DOI : 10.9775/kvfd.2024.33443 This study aimed to evaluate the effect of Low-level laser therapy (LLLT) and Boric acid (BA) or Borax decahydrate (BD) on the cell survival of gingival fibroblasts. Fibroblast planted plates were divided into 10 groups: Control group, BD 100 μg/mL (BD 100), BD 200 μg/mL (BD200), BA 100 μg/mL (BA100), BA 200 μg/mL (BA200), LLLT, LLLT+BD100 μg/mL (LLLT+BD100), LLLT+BD200 μg/mL (LLLT+BD200), LLLT+BA100 μg/mL (LLLT+BA100) and LLLT+BA 200 μg/mL (LLLT+BA200) groups. LLLT was performed at a dose of 4 J/cm2 for 160 sec. Following LLLT, MTT analysis was performed after the cells were kept in the incubator for 24 h. The LLLT+BA 200 group exhibited the highest cell density. In MTT analysis, significantly higher cell numbers were observed in the BA200, LLLT+BA100, and LLLT+BA200 groups compared to the control group (P<0.05). Then, the 3-(4,5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide assay and 8-hydroxy-2"-deoxyguanosine and Bcl-2 Associated X-Protein were analyzed. The 8-hydroxy-2"-deoxyguanosine and Bcl-2 Associated X-Protein levels were decreased in the LLLT+BA200 group compared to the control group (P<0.05). This study suggests that BA200, LLLT+BA100, and LLLT+BA200 increase the survival of fibroblast cells. Keywords : Borax decahydrate, Boric acid, MTT, Photobiomodulation, Photobiostimulation