¹Guru Angad Dev Veterinary and Animal Sciences University, College of Veterinary Science, Department of Veterinary Anatomy, Rampura Phul, Bathinda-151103, Punjab, INDIA DOI : 10.9775/kvfd.2024.33222 Limb joint diseases significantly reduce domestic animals" productive and reproductive capabilities. The present study was conducted to examine the anatomical features of the knee joint in cattle. Four naturally dead cattle of 2-3 years were collected and used in this study to record gross anatomical, radiographical characteristics and computed tomographic (CT) scans of the knee joints. Radiographs and CT scan images provided 2D and 3D visualization of the knee joint that was made up of articulatio antebrachiocarpea, articulatio intercarpea, and articulatio carpometacarpea. The synovial sacs of the intercarpal and carpometacarpal articulations were interconnected. The interosseous ligament connected the depression on the posterior margin of the distal radius with the intermediate and ulnar carpal bones. In the intercarpal articulation, one interosseous ligament was observed between the non-articular area on the distal surface of the intermediate carpal and the non-articular area of the fourth carpal. In the carpometacarpal joint, an interosseous ligament was observed connecting the nonarticular area on the lateral surface of the fused second and third carpals to the nonarticular area at the proximal end of the large metacarpal. The fibers of this ligament were blended with the interosseous ligament that connects the two carpal bones in the distal row. This study can help as an analytic tool for definitive diagnosis, prognosis, and research of the cattle knee joint. Keywords : Carpal bones, Cattle, Computed tomography, Knee joint, Ligament, Radiography