¹University of Sarajevo, Veterinary Faculty, Department of Clinical Sciences of Veterinary Medicine, 71000 Sarajevo, BOSNIA AND HERZEGOVINA
²University of Sarajevo, Faculty of Medicine, Department of Anatomy, 71000 Sarajevo, BOSNIA AND HERZEGOVINA 3 University of Banja Luka, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, 78000 Banja Luka, BOSNIA AND HERZEGOVINA
³University of Sarajevo, Faculty of Medicine, Department of Forensic Medicine, 71000 Sarajevo, BOSNIA AND HERZEGOVINA
⁴University of Sarajevo, Veterinary Faculty, Department of Basic Sciences of Veterinary Medicine, 71000 Sarajevo, BOSNIA AND HERZEGOVINA
⁵University of Banja Luka, Faculty of Science and Mathematics, Department of Zoology, 78 000 Banja Luka, BOSNIA AND HERZEGOVINA
⁶7 University of Sarajevo, Clinic for Orthopedics and Traumatology, Clinical Center University of Sarajevo, 71 000 Sarajevo, BOSNIA AND HERZEGOVINA
⁷Inovative Company ALBOS doo, 11 000 Belgrade, SERBIA
⁸University of Belgrade, Vinča Institute of Nuclear Sciences, 11 000 Belgrade, SERBIA DOI : 10.9775/kvfd.2024.32947 The aim of the research was to determine possible changes in the morphology of cells of the leukocyte order of peripheral blood, using geometric morphometric tests, after the application of calcium-aluminate and calcium-silicate cements to the dental pulp in rats. The study included 27 Wistar rats, divided into an experimental group (n=18) and a control group (n=9). Trepanation of the tip of the pulp cavity was performed, and placement of calcium-aluminate and calcium-silicate dental cements directly on the pulp. Peripheral blood samples were collected by vena caudalis puncture, with the aim of making blood smears. In the tpsUtil program, two-dimensional models of the examined leukocytes were created and they were converted into tps files, on which sixteen specific points were marked in the tpsDig program. We analyzed their shape in the MorphoJ program. The results of discriminant functional analysis determined that there was a statistically significant difference in the shape of the lymphocytes between the experimental animals, to which dental cements were applied, compared to the lymphocytes from the control group. Morphological differences were determined between the lymphocytes to which calcium aluminate and calcium silicate were applied. The results indicate that there were statistically significant morphological differences between these two groups of lymphocytes (P=0.02). The results obtained indicate the possibly unfavorable influence of the tested dental cements, especially calcium silicate, on leukocytic cells. Keywords : Biomaterials, Leukocyte cells, Dentin, Rat