¹Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box: 42805, Jeddah 21589, SAUDI ARABIA DOI : 10.9775/kvfd.2024.32444 Medicinal plants are rich in bioactive components, which exert various biological activities that are beneficial for living. This study evaluated the antioxidant, antitumor, and anti-inflammatory activities of Dhimran (Ocimum forsskaolii benth) in mice. The main volatile compounds in Dhimran essential oil (DEO) were endo-fenchol, tau-cadinol, and β-atlantol, while Dhimran extract (DE) was rich in caffeic acid and quercetin. These active compounds in DE and DEO possess antioxidant activity, scavenging 93% of DPPH radicals, antibacterial activity against MDR bacteria, and anticancer activity against HepG2 cancer cell line. These activities influenced the mice"s health. A total of 180 mice were divided into six treatment groups for 30 days as follows: T1 was fed a basal diet; T2 received CCl4 (187 mg/kg BW); T3 received DEO (4 mL/kg); T4 received DE (4 mL/kg); T5 received DEO+CCl4; and T6 received DE+CCl4. The oral administration of CCl4 to mice resulted in an increase in absolute liver weight (ALW) and relative organ weight (ROW) and a significant decrease in body weight gain and feed conversion ratio (FCR). Additionally, there was a notable reduction in levels of red blood cells (RBCs), hematocrit (Ht), hemoglobin (Hb), and platelets; however, there was an increase in white blood cells (WBCs). The administration of CCL4 in mice lowered total protein content; however, it raised the activity of AST, ALT, ALP, and LDH in mice liver homogenate compared to the control (P<0.05). CCl4 increased inflammation cytokines (TNF-α and IL-6). The coadministration of DEO or DE with CCl4 significantly corrected all biomarkers to control levels, with a preference for DE. Liver sections from the control, DE, and DEO groups revealed a normal structure. It is concluded that Dhimran extract or essential oil has antioxidant and anti-inflammatory properties that can be used as hepatoprotective agents against CCl4 toxicity. Keywords : Anticancer, Antioxidant, Anti-inflammatory, CCl4 toxicity, Essential oils, Hepatoprotective, Ocimum forsskaolii benth