¹Aydın Adnan Menderes University, Faculty of Veterinary Medicine, Internal Medicine Department, TR-09900 Aydın - TÜRKİYE DOI : 10.9775/kvfd.2024.31200 Mapping the skin with various biophysical properties can demonstrate not only skin condition but also organize a convenient approach to managing diseases. The aim of this study, mapping of the regional alteration for 6 biophysical parameters with noninvasive method. Totally 39 dogs [healthy (n=16) and canine atopic dermatitis (n=23)] were enrolled. Skin pH, hydration, melanin, sebum content and temperature for pinna, perilabial region, hind-fore feet (dorsal metacarpus-dorsal metatarsus), axillae, lateral thorax/flanks, elbows, abdomen/inguinal and ventral tail were measured. Atopic dogs exhibited more acidic skin (P<0.001) compared to healthy dogs, with the thorax/ flanks region showing significant regional differences (P=0.037). Hydration levels were consistently lower in atopic dogs (P<0.001), with significant regional differences observed in the abdominal/inguinal region (P=0.037). Skin temperatures were generally higher in atopic dogs, with a significant increase noted in the tail (P=0.037), while other areas showed no significant differences. Melanin and sebum content did not exhibit significant regional variations. In conclusion, this study demonstrated regional variations in biophysical parameters in atopic dogs using non-invasive measurements. These findings provide valuable insights for understanding canine atopic dermatitis and its management. Keywords : Atopic dog, Non-invasive measurement, Skin-barrier dysfunction, Skin hydration, Skin pH