Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2024 , Vol 30 , Issue 1
Synergistic and Dose-Dependent Effects of Pinostrobin, Pinocembrin and Pinobanksin on Different Breast Cancer Cell Lines
Abdullah MELEKOĞLU1, Ayşe Begüm CEVİZ2, Allison Pınar ERONAT2, Tülin ÖZTÜRK5, Funda PEHLEVAN2, Hülya YILMAZ-AYDOĞAN2, Oğuz ÖZTÜRK2
1Pamukkale University, Faculty of Science and Letters, Department of Biology, TR-20160 Denizli - TÜRKİYE
2Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Molecular Medicine, TR-34093 İstanbul - TÜRKİYE
3Istanbul Health and Technology University, Faculty of Medicine, Department of Medical Genetics, TR-34445 İstanbul - TÜRKİYE
4Haliç University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, TR-34060 İstanbul - TÜRKİYE
5Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Pathology, TR-34098 İstanbul - TÜRKİYE
6Department of Medical Laboratory Techniques, Vocational School of Health Services, Istanbul Gelisim University, TR-34310 İstanbul - TÜRKİYE DOI : 10.9775/kvfd.2023.30187 In this study, the cytotoxic and apoptotic effects of three phenolic compounds highly found in the poplar type propolis; pinostrobin (PS), pinocembrin (PC) and pinobanksin (PB), were investigated individually and in combination on hormon-positive (MCF-7) and triple negative (MDA-MB-231) breast cancer cell lines and fibrocystic breast epithelium (MCF-10A) as control. Assessment of cytotoxicity and apoptosis were performed with WST-1 and Annexin V-7AAD assays, respectively. All statistical analyses including the two-way ANOVA and multiple-t-test were performed using GraphPad Prism software. Individually, PB (P<0.0001), PS (P<0.0001), and PC (P<0.05) demonstrated potent cytotoxic effects at moderate to high doses and late time intervals on MCF-7. PB and PS have been found to have a significant proliferative effect at low doses (P<0.0001), however, this effect disappeared in higher doses in this cell line. Dual combinations of PB+PC and PB+PS were toxic on MCF-10A, however, dual combination of PS+PC and the triple combination (PB+PS+PC) showed no cytotoxicity until high doses at late time intervals (P>0.05). On MCF-7, the triple combination induced cytotoxic/apoptotic effects even with the 25% dose and 50% dose on MDA-MB-231(p<0.0001). Our findings clearly showed that different combinations of these phenolic substances can have synergistic cytotoxic effects and even hormetic effects in non-tumorogenic cells. Keywords : Breast cancer cell line, Cytotoxicity, Pinobanksin, Pinocembrin, Pinostrobin, Propolis