¹Shihezi University, Collage of Animal Science & Technology, Xinjiang 832003, P. R. CHINA DOI : 10.9775/kvfd.2022.27091 Earthworm antimicrobial peptides combined with probiotics were supplemented to the yellow-feathered broiler diet, and the cecal intestinal flora was subjected to 16S rDNA Qualcomm sequencing. The results showed that the total number of species in S1 (control group) was significantly higher than S2 (earthworm antimicrobial peptides) and S3 (Compound probiotic preparation), S4 (earthworm antibacterial peptide + composite probiotic preparation) (P<0.05), the colony structure of S1 is significantly different from S2, S3, and S4 (P<0.05). At the phylum level of each test group, the dominant bacterial groups were Firmicutes, Bacteroides, and Proteobacteria. Among them, S2, S3, and S4 were significantly higher than S1 in the relative abundance of Firmicutes and Bacteroides (P<0.05), the relative abundance of Proteobacteria was significantly lower than S1 (P<0.05). At the genus level, S2, S3, and S4 were significantly higher than S1 in the relative abundance of Lactobacillus (P<0.05), where S4 is higher than S3 (P>0.05). Earthworm antimicrobial peptides combined with compound probiotics can increase the number of beneficial bacteria and reduce the number of harmful bacteria to regulate intestinal flora, indicating that earthworm antimicrobial peptides combined with compound probiotics can be used as new green antibiotics in animal production. Keywords : Earthworm antibacterial peptides, Probiotic, 16S rDNA