Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2022 , Vol 28 , Issue 3
Effects of Oxidative Stress-Related Major Molecular Parameters on Milk Composition in Weaning Period of Damascus Goats
Hüseyin ÖZKAN1, Sevda DALKIRAN2, İrem KARAASLAN3, Ufuk KAYA4, Baran ÇAMDEVİREN2, Murat YÜKSEL5, Akın YAKAN1
1Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Genetics, TR-31060 Hatay - TÜRKİYE
2Hatay Mustafa Kemal University, Institute of Health Sciences, Department of Molecular Biochemistry and Genetics, TR- 31060 Hatay - TÜRKİYE
3Hatay Mustafa Kemal University, Technology and Research & Development Center (MARGEM), TR-31060 Hatay - TÜRKİYE
4Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Biostatistics, TR-31060 Hatay - TÜRKİYE
5Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-31060 Hatay - TÜRKİYE DOI : 10.9775/kvfd.2022.27101 Th e weaning process in goat breeding is applied in various methods depending on the breeding administration. In this study, blood and milk samples have been collected from Damascus goats at the weaning day (postpartum 105th day-weaned day) and a week later (post-weaned day). In addition to determining cortisol and MDA in plasma, COX-2 and NFE2L2 activities have been investigated both mRNA and protein levels from leucocytes and plasma. Compositional parameters of milk have also been analyzed and the possible relations between studied parameters have been investigated. While expression levels of COX-2 and SCC of milk decreased, pH of milk was increased in post-weaned day samples. Th e MDA, FFDM, protein, lactose and freezing point were decreased in the post-weaned day. Milk fat was negatively correlated with NFE2L2, and milk protein had positively correlated with SCC and FFDM. On the other hand, lactose was positively correlated with FFDM and protein. In addition, most of the compositional parameters positively correlated with a freezing point; they were negatively correlated with electrical conductivity. According to the results obtained from the study, it is thought that the decrease in milk fat in goats may be an indicator of increased oxidative stress in lactating goats due to the increase in NFE2L2 protein, which has a central role in the antioxidant response. Keywords : COX-2, Damascus goat, Gene expression, NFE2L2, Weaning in goats