Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2022 , Vol 28 , Issue 2
Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm
Burcu USTUNER1, Selim ALCAY1, Elif GOKCE2, M. Melih YILMAZ1, Ahmet AKTAR1, Oguzhan HURAYDIN1, Muhammed DUMAN3, N. Tekin ONDER4, Eser AKAL5, Zekariya NUR1
1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR-16059 Görükle, Bursa - TÜRKİYE
2Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Namik Kemal University, TR-59030 Tekirdağ - TÜRKİYE
3Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Uludag University, TR-16059 Görükle, Bursa - TÜRKİYE
4Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kafk as University, TR-36300 Paşaçayırı, Kars - TÜRKİYE
5Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ondokuz Mayis University, TR-55200 Atakum, Samsun - TÜRKİYE DOI : 10.9775/kvfd.2021.26855 Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. Th e highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P<0.05). Th ere was no significant diff erence in the rate of post-thaw defective acrosomes when the other extender groups were compared with the control (P>0.05). Th e highest percentage of post-thaw DNA fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant diff erent from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can be used successfully for freezing ram semen. Keywords : Cryopreservation, Lyophilized extender, Rainbow trout seminal plasma, Ram semen