Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 6
Calcium (Ca2+) Oscillations and Intensity in Fresh Embryo and Vitrified Embryos Produced from Intra-cytoplasmic Sperm Injection (ISCI)
Widjiati WIDJIATI1, Zakiyatul FAIZAH2, Viski Fitri HENDRAWAN3, Helly Nurul KARIMA4, Choirunil CHOTIMAH4, Sutiman Bambang SOEMITRO5, A. A. Muhammad Nur KASMAN6, Epy Muhammad LUQMAN
1Universitas Airlangga, Faculty of Veterinary Medicine, Department of Veterinary Science, 60115 Surabaya, INDONESIA
2Universitas Airlangga, Faculty of Medicine, Department of Biomedical Science, 60132 Surabaya, INDONESIA
3Universitas Brawijaya, Faculty of Veterinary Medicine, Department of Reproduction, 65151, Malang, INDONESIA
4Universitas Brawijaya, Bio-Science Central Laboratory, 65151, Malang, INDONESIA
5Universitas Brawijaya, Faculty of Science, Department of Biology, 65151, Malang, INDONESIA
6Universitas Muhammadiyah Mataram, Faculty of Health Science, 83115, Mataram, INDONESIA
DOI : 10.9775/kvfd.2021.26332 This study aims to determine the intracellular calcium profile and viability of embryos produced by the Intra-Cytoplasmic Sperm Injection (ICSI) method. In this study, there were 2 groups (T1: fresh embryos, T2: embryos posts vitrification). The stages of the study included medium preparation, goat oocyte collection, in vitro maturation of Kacang goat oocytes, fertilization using the ICSI method, and examination of the calcium (Ca2+) intensity profile of fresh embryos and embryos post vitrification per unit time (sec). Measuring the intensity of Ca2+ using a Confocal Laser Scanning Microscope (CLSM) with time-lapse, taken at 3 points, namely point 1: edge, point 2: middle, and point 3: edge of the embryo sample. The fertilized embryos showed that the average calcium intensity of T1 was 334.62±8.60 and T2 was 408.2±13.67. The intensity of Ca2+ in embryos post vitrification is higher than that of in fresh embryos. The oscillation of Ca2+ in fresh embryos was in tune to the measurement point of 50 sec, while in embryos post vitrification the intensity from the 10th, 20th early sec and the 50th end interval were not consistent. It can be concluded that the intensity of Ca2+ in embryos post vitrification is higher than that of in fresh embryos. The dynamics of Ca2+ in frozen embryos experiencing changes in intensity indicatie a change in embryo quality due to vitrification. Keywords : Food production, Calcium, Embryo, Freezing, ICSI, Oscillation