Kafkas Üniversitesi Veteriner Fakültesi Dergisi Articles in Press
The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
Hüseyin ÖZDEN1, Gökhan KARACA2, Huri BULUT3, Mehmet NİYAZ4, Muhammed GÖMEÇ5, Çağatay E. DAPHAN2
1Ahi Evran University Faculty of Medicine Department of General Surgery, TR-40100 Kırşehir - TURKEY
2Kırıkkale University Faculty of Medicine Department of General Surgery, TR-71450 Kırıkkale - TURKEY
3İstinye University Faculty of Medicine Department of Medical Biochemistry, TR-34460 Istanbul - TURKEY
4Başkent University Faculty of Medicine Department of Medical Genetics, TR-06790 Ankara - TURKEY
5Cumhuriyet University Faculty of Medicine Department of General Surgery, TR-58140 Sivas - TURKEY
DOI : 10.9775/kvfd.2021.25935 Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs. Keywords : Anastomotic leakage, Colonic anastomosis, Hydroxyproline, İschemia reperfusion injury, Mesenchymal stem cell