Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2021 , Vol 27 , Issue 5
The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats
Levent SAHIN1, Erdem TOKTAY2, Muhammed YAYLA3, Pınar AKSU KILICLE4, Ahmet HARMANKAYA5, Cem OZIC6, Serdar YIGIT7, Nevra AYDEMIR CELEP8
1Kafkas University, Faculty of Medicine, Emergency Medicine Department, TR-36100 Kars - TURKEY
2Kafkas University, Faculty of Medicine, Histology and Embryology Department, TR-36100 Kars - TURKEY
3Kafkas University, Faculty of Medicine, Pharmacology Department, TR-36100 Kars - TURKEY
4Kafkas University, Faculty of Medicine, Biology Department, TR-36100 Kars - TURKEY
5Kafkas University, Faculty of Medicine, Biochemistry Department, TR-36100 Kars - TURKEY
6Kafkas University, Faculty of Medicine, Medical Biology Department, TR-36100 Kars - TURKEY
7Kafkas University, Faculty of Medicine, Histology and Embryology Department, TR-36100 Kars - TURKEY
8Ataturk University, Faculty of Medicine, Histology and Embryology Department, TR-25100 Erzurum - TURKEY DOI : 10.9775/kvfd.2021.25880 We hypothesized that dragon fruit extract (DFE) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat intestine. The rats used in the study were randomly divided into 8 groups, with 6 animals in each group. Groups 1 and 2 were not exposed to ischemia. Ischemia was induced for 1 h in groups 3, 4, 5, 6, 7 and 8. The clamps were then removed to allow reperfusion for 45 min (Groups 6, 7 and 8). DFE was given to rats at doses of 500 mg/kg and 1000 mg/kg 30 min before Ischemia (I) and Ischemia/Reperfusion (I/R) administration. At the end of the experiment, histopathological, biochemical and molecular analyses were performed on the intestinal tissues. While glutathione peroxidase, superoxide dismutase, glutathione levels increased significantly in the I+DFE500, I+DFE1000, I/R+DFE500 and I/R+DFE1000 groups compared to the I and I/R groups, there was a significant decrease in tumor necrosis factor -α, Caspase 3 and malondialdehyde (P<0.05). In addition, the loss of tissue integrity, and the increase of infl ammatory cells were decreased in the treatment groups. As a result, it was determined that DFE has a strong protective role against oxidative damage in the treatment of intestinal ischemia-reperfusion injury. Keywords : Dragon fruit extract, Intestine, Ischemia/reperfusion, Oxidative stress, Rat