¹College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, CHINA
²College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 625014, CHINA DOI : 10.9775/kvfd.2021.25681 Cryptosporidium muris is a zoonotic protozoan. Characterization of genetic diff erentiation can be exploited to trace the origins of the parasite. A Cryptosporidium isolate from brown rat was obtained by microscopy and genetically characterized. C. muris was identified with minor nucleotide diff erences. Further subtype analysis was performed using a multilocus sequence typing (MLST) tool. At each locus, the C. muris isolate was confirmed to be M12, M4, M1 and M2 subtype, representing as a new MLST subtype. Therefore, the results suggest that there was probably a new source of infection for C. muris in the study region. Keywords : Cryptosporidium muris, 18S rRNA, MLST, Subtype, Brown rat