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Kafkas Üniversitesi Veteriner Fakültesi Dergisi
2020 , Vol 26 , Issue 4
Effects of Semen Extender Supplemented with Bovine Serum Albumin (BSA) on Spermatological Traits of Saanen Buck Semen Stored at +4°C
1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University - Cerrahpasa, TR-34320 Avcilar, Istanbul - TURKEY
DOI :
10.9775/kvfd.2019.23674
The aim of this study was to investigate the effects of two modified semen diluents on the spermatological parameters of Saanen goat
sperm stored at 4°C. Ejaculates were obtained from five Saanen bucks by means of an electro-ejaculator and divided into five aliquots. Then,
aliquots of ejaculates were used to create five experimental groups as follows: Group 1 (control), group 2A containing seminal plasma (SP)
and bovine serum albumin (BSA; 10 mg/mL-1), group 2B containing BSA without SP, group 3A containing egg yolk (EY; 17.00%) and SP and
finally group 3B containing EY without SP. All aliquots of semen were extended with Tris-based extender. Spermatological parameters of
experimental groups were observed at 0, 6, 12 and 24 h periods during storage at 4°C. Motility of semen in groups 2A and 2B was found to be
best values compared to other groups. However, the motility of group 3A at 24 h was 0.00%. In conclusion, it was concluded that the addition
of 10 mg/mL-1 of BSA to Tris-based extender could be useful in the storage of Saanen goat semen regardless of SP at 4°C.
Keywords :
Bovine serum albumin, BSA, Extender, Goat semen, Short term storage