¹Afyon Kocatepe University, Veterinary Faculty, Department of Food Hygiene and Technology, TR-03200 Afyonkarahisar - TURKEY
²Afyon Kocatepe University, Veterinary Faculty, Department of Pharmacology and Toxicology, TR-03200 Afyonkarahisar - TURKEY
³Afyon Kocatepe University, Bayat Vocational School, TR-03780 Bayat, Afyonkarahisar - TURKEY
⁴Afyon Kocatepe University, Veterinary Faculty, Department of Biochemistry, TR-03200 Afyonkarahisar - TURKEY
⁵Afyon Kocatepe University, Veterinary Faculty, Department of Physiology, TR-03200 Afyonkarahisar - TURKEY DOI : 10.9775/kvfd.2019.22401 The present study aimed to evaluate the persistence of kanamycin in lactating Anatolian buffalo milk followed by an intramuscular injection of kanamycin. The collection of milk samples was performed twice daily up to the 10th milking followed by kanamycin injection and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was employed for the residue analysis. The detection limit of the method was determined as 3.56 μg/kg. The highest concentrations of kanamycin were determined in the first milking after injection and mean concentration of this milking was found to be as 1473 μg/kg. Kanamycin residue in all buffalo milk samples was lower than the maximum residue limit (150 μg/kg) with the fifth milking. In addition, a monitoring study was conducted to determine whether buffalo milk marketed in Afyonkarahisar pose a public health risk regarding kanamycin residue. The results of the monitoring study showed that one (2%) of the 50 buffalo milk samples contained kanamycin at the concentration of 22.36 μg/kg. In conclusion, this study determined the persistence of kanamycin in Anatolian buffalo milks based on an LC-MS/MS method. In addition, results of the study showed that buffalo milks marketed in Afyonkarahisar Province pose a very low risk regarding kanamycin residue. Keywords : Kanamycin, Aminoglycosides, Anatolian buffaloes, Milk, LC-MS/MS