¹College of Animal Science, Tarim University, Aral 843300, P.R. CHINA
²Animal Loimia Controlling and Diagnostic Center of Aksu Region, Aksu, Xinjiang 843300, P.R. CHINA
³Animal husbandry and veterinary workstations of Barkol kazak autonomous county, Kumul 839200, P.R. CHINA DOI : 10.9775/kvfd.2018.21125 The direct damage and spread of pathogens of Rhipicephalus (Boophilus) microplus to cattle -based livestock is extremely serious, the traditional chemical acaricides control have many disadvantages, and vaccine prevention and control is a potential alternative. The commercially available vaccine, based on Rhipicephalus (Boophilus) microplus Bm86, has been favored by farmers and veterinarians in some areas, but it also has drawbacks such as reduced effectiveness due to genetic variation. Based on the fact that Bm91 and Bm86 sharing can enhance the immune effect of Bm86, as well as the characteristics of DNA vaccines, the combination of DNA vaccine and protein vaccine enhanced the immune effect. In this study, adopts the local Rhipicephalus (Boophilus) microplus strains, using prokaryotic expression system preparation Bm86 and Bm91 proteins, and using the eukaryotic expression vector pVAXl containing the CpG sequences constructed Bm86 and Bm91 double gene eukaryotic expression system. This research provides materials for the next step of Co-immunizing animals with Bm86 and Bm91 mixed proteins with Bm86 and Bm91 dual-gene carriers, it also provides a way for co-immunization with multi-antigen protein and multi-gene carriers to control ticks. Keywords : Rhipicephalus (Boophilus) microplus, Bm86, Bm91, Co-expression