Molecular Screening and Characterization of Shiga Toxin-producing Escherichia coli By Multiplex PCR Assays for stx1, stx2, eaeA, H7 in Raw Milk

Koycho KOEV; Georgi ZHELEV; Plamen MARUTSOV; Krasimira GOSPODINOVA; Vladimir PETROV; Todor STOYANCHEV

doi:10.9775/kvfd.2018.20373

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Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2019 , Vol 25 , Issue 2

Molecular Screening and Characterization of Shiga Toxin-producing Escherichia coli By Multiplex PCR Assays for stx1, stx2, eaeA, H7 in Raw Milk

Koycho KOEV¹, Georgi ZHELEV¹, Plamen MARUTSOV¹, Krasimira GOSPODINOVA¹, Vladimir PETROV¹, Todor STOYANCHEV²

¹Trakia University, Department of veterinary microbiology, infectious and parsitic diseases, Stara Zagora, BULGARIA
²Trakia University, Department of Food Hygiene, Technology and Control, Veterinary Legislation and Management, Stara Zagora, BULGARIA DOI : 10.9775/kvfd.2018.20373 The presence of Shiga-toxin producing Escherichia coli (STEC) was evaluated in bulk milk from dairy cattle farms in Bulgaria. From 20 regional farms only 4 were selected as a source for milk sampling on the basis of their annual STEC shedding in feces. All the samples were PCR analyzed after selective enrichment. Positive samples were detected in subsequent molecular testing as: two of samples (9.08%) were positive for stx1, one sample (4.54%) for stx2, 10 samples (45.54%) for eae and 12 (54.54%) for H7 coding genes. Keywords : Escherichia coli O157: Н7, Dairy farms, Bulk milk, Enrichment, PCR

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