¹İstanbul Kültür University, Nutrition and Dietetics, TR-34192 Bahçelievler, İstanbul - TURKEY
²İstanbul University-Cerrahpaşa, School of Veterinary Medicine, Department of Food Hygiene and Technology, TR-34320 Avcılar, İstanbul - TURKEY
³İstanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine, Department of Clinical Microbiology, TR-34098 Cerrahpaşa, İstanbul - TURKEY DOI : 10.9775/kvfd.2018.20702 In this study, it is aimed to analyze the risk factors related to Listeria monocytogenes and Esherichia coli O157:H7 monitoring in fresh kashar cheese and milk creme in the province of Istanbul. It has 100 pieces of fresh kashar cheese and 100 pieces of milk creme material. Samples were confirmed by PCR methods and genetic basis with conventional microbiological sowing methods and analysis methods. Another case for the study is to obtain significant epidemiological environment for the field strains in the country in terms of L. monocytogenes and E. coli OH157: H7, where high polymorphic protein structures and rapid genetic variation exist. In the study, 4 (2%) of E. coli OH157: H7 and 6 (3%) of L. monocytogenes were found among 200 milk slices and fresh kashar cheese samples. Those microorganisms can easily reproduce in natural environment such as soil and silage and with primer/secondary contamination, they appear to be health threatening food related pathogens. It is suggested to have studies for optimal food safety and this can be provided in accordance with appropriate legislation. Keywords : PCR, Food safety, L. monocytogenes, E. coli