¹Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY
²Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY
³Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Balikesir University, TR-10463 Balikesir - TURKEY DOI : 10.9775/kvfd.2018.20222 The aim of this study was to determine the oxidative/nitrosative stress, leptin and progesterone concentrations in pregnant and non-pregnant Abaza goats after synchronization with controlled internal drug release (CIDR) during the breeding season. For this purpose, 40 clinically healthy Abaza goats, aged 2-4 years, were intravaginally exposed to CIDR on day 0, and injected with equine chorionic gonadotropin and prostaglandin F2α on day 9 of the experiment. CIDR was removed on day 11. At the end of the experiment, they were monitored for estrus and exposed to fertile males for mating. Blood samples were collected 8 days before synchronization, then on days 0, 11 of CIDR insertion and on mating day. A pregnancy diagnosis was conducted using transrectal ultrasonography 30 day after mating. The blood serum from 40 goats (30 pregnant + 10 non-pregnant) was used for biochemical analyses. Malondialdehyde (MDA), nitric oxide (NO), total oxidant capacity (TOC), total antioxidant capacity (TAC) and endothelial NO synthase (eNOS) activities were significantly higher on mating day in pregnant goats compared to non-pregnant goats (P<0.05). The eNOS activity and TOC concentrations were significantly higher on day 11 in pregnant goats compared to non-pregnant goats (P<0.001). Serum P4 concentration increased in pregnant group on day 11 and decreased all groups on mating day than day -8, day 0 and day 11 (P<0.001). In conclusion, the administration of CIDR to Abaza goats exacerbated oxidative and nitrosative stress. Keywords : Abaza goat, Synchronization, eNOS activities, Leptin, Nitric oxide, Progesterone