¹Mehmet Akif Ersoy University Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-15030 Burdur - TURKEY
²Ankara University Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-06110 Ankara - TURKEY
³Ankara University Faculty of Veterinary Medicine, Department of Biostatistics, TR-06110 Ankara - TURKEY
⁴Padova University Faculty of Veterinary Medicine Department of Animal Medicine, Production and Health, ITALY DOI : 10.9775/kvfd.2017.17181 The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4°C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x106 group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4°C. Keywords : Bull sperm, Cholesterol, Motility, Short term storage, 7-dehydrocholesterol