¹Department of Animal Science, Faculty of Agriculture. Islamic University of Kalimantan, Banjarmasin, South Kalimantan, INDONESIA
²Officer of Animal husbandry, District of Balangan, Province South Kalimantan, INDONESIA DOI : 10.9775/kvfd.2017.17556 This study was conducted to investigate the effect of fish oil addition to the semen extender on post-thaw quality of Bali bull spermatozoa. Fish oil was added at the doses of 0 (control), 50, 100, 150 and 200 mg/100 mL to the skim milk-egg yolk extender. Supplementation of fish oil at the dose of 100 mg/mL significantly (P<0.05) increased both live sperm rate and motility, but its dose of 200 mg/mL caused a significant (P<0.05) decrease in the live sperm rate when compared with the control group. A significant (P<0.05) increment was observed in abnormality rate of frozen-thawed spermatozoa in comparison with the control group when all doses of fish oil except 50 mg/100 mL were added. In conclusion, addition of 100 mg fish oil to 100 mL of skim milk-egg yolk extender could be beneficial for the improvement of the quality of Bali bull spermatozoa after freeze-thawing. Keywords : Boğa sperması, Balık yağı, Kriyoprezervasyon, Canlı sperm, Motilite