Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2017 , Vol 23 , Issue 3
An Investigation on Protective Effect of Viburnum opulus L. Fruit Extract Against Ischemia/Reperfusion-Induced Oxidative Stress After Lung Transplantation in Rats
EKEN A1, YÜCEL O2, BOŞGELMEZ İİ1, BALDEMIR A3, ÇUBUK S2, ÇERMIK AH4, ÜNLÜ ENDIRLIK B1, BAKIR E1, YILDIZHAN A2, GÜLER A5, KOŞAR M6
1Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, TR-38039 Melikgazi, Kayseri - TURKEY
2Department of Thoracic Surgery, Gulhane Military Medical Academy, TR-06010 Keçiören, Ankara - TURKEY
3Department of Pharmaceutical Botany, Faculty of Pharmacy, Erciyes University, TR-38039 Melikgazi, Kayseri - TURKEY
4Pathology Service, Etimesgut Military Hospital, TR-06810 Etimesgut, Ankara - TURKEY
5Department of Cardiac and Vascular Surgery, Gulhane Military Medical Academy, TR-06010 Keçiören, Ankara - TURKEY
6Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, TR-38039 Melikgazi, Kayseri - TURKEY DOI : 10.9775/kvfd.2016.16964 The aim of this study was to investigate the protective effect of Viburnum opulus L. fruit extract against ischemia/reperfusion (I/R)-induced oxidative stress during the lung transplantation. For this purpose, 30 female rats were firstly randomized to form of donor and recipients. After then, the rats were divided into three groups named as control, I/R, and V. opulus + I/R. Experimental rats were subjected to lung transplantation with ischemia followed by 2 h of reperfusion. Transplantation-related lung injury was evaluated by multiple parameters. A significant decrease was observed in the enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), and the levels of glutathione and total antioxidant status (TAS), whereas the levels of malondialdehyde (MDA), total oxidant status (TOS), and protein carbonyl were significantly increased in lung tissue samples of I/R group in comparison to the control group. However, treatment with V. opulus fruit extract resulted in significant reduction of MDA and protein carbonyl levels and increment of the antioxidant system. In conclusion, V. opulus fruit extract showed protective effects against I/R-induced oxidative stress during lung transplantation probably by the radical scavenging and antioxidant activity. Therefore, this fruit extract can be efficient in the prevention of I/R-related lung toxicity. Keywords : Oxidative Stress, Transplantation, Ischemia/Reperfusion, Antioxidant