¹Department of Pathology, Faculty of Veterinary Medicine, Balikesir University, TR-10145 Balikesir - TURKEY
²Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY
³Department of Biochemistry, Faculty of Art and Science, Kafkas University, TR-36100 Kars - TURKEY
⁴Department of Emergency, Faculty of Medicine, Kafkas University, TR-36100 Kars - TURKEY
⁵Department of Physiology, Faculty of Medicine, Trakya University, TR-22030 Edirne - TURKEY DOI : 10.9775/kvfd.2016.15862 Omega-3 is a polyunsaturated fatty acid known to have immunomodulatory functions. In the present study, ameliorative potential of omega-3 in experimental carbon tetrachloride (CCl4) toxicity was investigated. Total of 40 adult male Wistar albino rats were allocated into five groups and were subcutaneously given once every two days for 6 weeks the followings: Group 1 (Control): 0.5 ml/kg serum physiologic, Group 2 (Omega): 0.5 g/kg omega-3, Group 3 (Vehicle): 0.5 ml/kg pure olive oil, Group 4 (CCl4): 0.5 ml/kg CCl4, Group 5 (CCl4 + Omega): 0.5 ml/kg CCl4 plus 0.5 g/kg omega-3. At the end of the treatments, blood samples were collected and necropsy was performed for collection of liver tissues. Serum AST, ALT, GGT, TAC, TOC, triglyceride, and visfatin levels were detected. Liver morphology and immunoreactivities against TGF-α, TGF-β, PPAR-α, and PPAR-γ were assessed. Serum AST, ALT, GGT, and TOC levels significantly increased while TAC level decreased in CCl4 given animals as compared to the control group. No significant changes were observed in triglyceride and visfatin levels. Immunohistochemical staining revealed increased TGF-α and TGF-β expressions and decreased PPAR-α and PPAR-γ expressions in liver of CCl4 given animals. Omega-3 supplementation has prominent effects in correcting the biochemical and immunohistochemical parameters studied as well as the tissue morphology. The results of the investigation indicated that omega-3 has ameliorative effects on the oxidative tissue degeneration and inflammatory processes induced by CCl4 treatment in rats Keywords : CCl4, Omega-3, TGF-α, TGF-β, PPAR-α, PPAR-γ